Gibco™

Bac-to-Bac™ Vector Kit

The Bac-to-Bac™ Vector Kit contains a pFastBac™ 1 vector, as well an expression control vector, intended for use as part자세히 알아보기

The Bac-to-Bac™ Vector Kit contains a pFastBac™ 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac™ Baculovirus Expression System (Cat. No. 10359-016), which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac™ System relies on generation of recombinant baculovirus by site-specific transposition in _E. coli_rather than homologous recombination in insect cells (Figure 1). This system features:

• Time-saving expression bacmid. With Bac-to-Bac™, the expression cassette of the pFastBac™ vector recombines with the parent bacmid in DH10Bac™ E. coli Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.
• Easy colony screening. The parent bacmid in DH10Bac™ _E. coli_contains a segment of the _lac_Zα gene. The _lac_Zα gene is disrupted upon transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.
The Bac-to-Bac™ Baculovirus Expression System is designed for fast, small-scale production of recombinant baculovirus. The pFastBac™ 1 vector provided by this kit offers the strong polyhedrin promoter for protein expression and a large multiple cloning site for simplified cloning.

사양

프로모터Polyhedrin

제품 유형Vector Kit

단백질 태그Untagged

클로닝 방법Restriction Enzyme/MCS

수량1 kit

벡터pFastBac

제품라인Bac-to-Bac™

Unit SizeEach