Thermo Fisher Scientific AF9 Polyclonal Antibody
Rabbit polyclonal antibody targeting human AF9 (MLLT3) protein. Validated for Western blot and immunocytochemistry. High specificity and affinity purified via antigen chromatography. Provided in liquid form with glycerol for stability. Suitable for res...
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Applications
Western Blot (WB)
- Tested Dilution: 0.04–0.4 µg/mL
- Publications: View 1 publication
Immunocytochemistry (ICC/IF)
- Tested Dilution: 0.25–2 µg/mL
- Publications: View publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human MLLT3. Recombinant protein control fragment (Product #RP-101898) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789133 |
Product Specific Information
Immunogen sequence:
SSKSDSEQPS PASSSSSSSS SFTPSQTRQQ GPLRSIMKDL HSDDNEEESD EVEDNDNDSE MERPVNRGGS RSRRVSLSDG SDSESSSASS PLHHEPPPPL LKTNNNQILE VKSPIKQSKS DKQIKNGECD KAYLDELVEL HRRLMTLR
Target Information
The human AF9 gene is one of the most common fusion partner genes with the ALL1 (MLL) gene at 11q23, resulting in t(9;11)(p22;q23).
AF9 spans over 100 kb and contains two patient breakpoint cluster regions (BCRs):
- BCR1: within intron 4 (site A)
- BCR2: spans introns 7 and 8 (site B)
Structural features include:
- In vivo DNA topo II cleavage site and in vitro DNase I hypersensitive site in intron 7 (BCR2)
- Scaffold associated regions (SARs): SAR1 in intron 4 and SAR2 encompassing exons 5–7
These regions share structural similarities with MLL BCR, supporting a DNA breakage and repair model for nonhomologous recombination between MLL and AF9.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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