Thermo Fisher Scientific EDD1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human E3 Ubiquitin Protein Ligase (EDD1). Suitable for WB, IHC, and IP applications. Unconjugated liquid form, 1 mg/mL concentration. Recommended storage at 4°C and shelf life of 1 year.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 600 and 650 of human E3 Ubiquitin Protein Ligase, HECT domain containing, 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
EDD (E3 identified by Differential Display) is a progestin-regulated gene isolated from T-47D human breast cancer cells.
Based on sequence homology, EDD appears to be the human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor required for imaginal disc growth.
EDD contains a HECT domain in the carboxy terminus, functioning as an E3 ubiquitin-protein ligase.
EDD has been shown to bind ubiquitin and may act as an E3 ubiquitin-protein ligase similar to other HECT family proteins.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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