Thermo Fisher Scientific Bcl-2 Monoclonal Antibody (Bcl2/100), FITC
인간 Bcl-2 단백질을 인식하는 FITC 결합 단클론 항체로, Flow Cytometry에 최적화됨. 항원은 인간 Bcl-2의 아미노산 41-54 서열 기반 합성 펩타이드. 항체는 액상 형태로 제공되며, 4°C 암소 보관. 세포 사멸 연구 및 항암 메커니즘 분석에 활용 가능.
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Thermo Fisher Scientific Bcl-2 Monoclonal Antibody (Bcl2/100), FITC
Applications
- Flow Cytometry (Flow)
Tested Dilution: 4 µL / 1×10⁶ cells
View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Bcl2/100 |
| Immunogen | Synthetic peptide corresponding to amino acids 41–54 of human Bcl-2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534544 |
Additional Formats
Product Specific Information
This antibody recognizes Bcl-2, a 26 kDa intracellular proto-oncogene with anti-apoptotic effect.
It is expressed in the outer mitochondrial membrane, endoplasmic reticulum, and nuclear envelope.
Target Information
BCL-2 is a key regulator of apoptosis that can inhibit or promote cell death.
Members of the BCL-2 family form dimers to modulate apoptosis.
- Bag1 forms a heterodimer with BCL-2, enhancing its anti-apoptotic effect.
- Bax and Bak promote cytochrome c release, initiating apoptosis.
Constitutive expression of BCL-2, such as through translocation to the Ig heavy chain locus, is associated with follicular lymphoma.
In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas normal centers are negative.
Two transcript variants of BCL-2 differ in their C-terminal ends.
Overexpression of BCL-2 has been linked to B-cell lymphoma and prostate cancer.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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