Thermo Fisher Scientific alpha-1 Antitrypsin Polyclonal Antibody
Thermo Fisher Scientific의 알파-1 안티트립신 폴리클로날 항체는 인간 및 마우스 시료에 반응하며, WB, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. 고순도의 Affinity Chromatography 정제 항체로, 안정적인 PBS/glycerol buffer에 보관됩니다. 연구용 전용 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 156–255 of human Alpha-1 Antitrypsin (SERPINA1) (NP_000286.3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 4.1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804340 |
Product Specific Information
Immunogen sequence:
EDVKKLYHSE AFTVNFGDTE EAKKQINDYV EKGTQGKIVD LVKELDRDTV FALVNYIFFK GKWERPFEVK DTEEEDFHVD QVTTVKVPMM KRLGMFNIQH
Positive Samples: SW480, HT-29, Mouse lung
Cellular Location: Endoplasmic reticulum, extracellular matrix, extracellular space
Target Information
Alpha-1-Antitrypsin (Alpha-1-AT) is synthesized in the liver and functions as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung.
In the absence of alpha-1-AT, these enzymes are not inhibited and may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema), hepatic cirrhosis in infants, and respiratory distress of the newborn.
Increased alpha-1-AT levels occur as an acute phase response to tissue necrosis and inflammation. Elevated serum levels are observed in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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