Thermo Fisher Scientific Non-Phospho-Paxillin (Tyr118) Monoclonal Antibody (GT722)
Non-Phospho-Paxillin (Tyr118) 단일클론 항체(GT722)는 인간, 마우스, 랫트 시료에 반응하며 Western blot 및 면역형광에 적합합니다. Paxillin의 비인산화형 검출에 사용되며, 단백질 발현 및 세포 부착 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT722 |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human Paxillin (non-phospho Tyr118) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787189 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Chicken (100%).
- Positive Control: A431, HeLa, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, Cyclin D1-knockout HeLa.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain, independent of the extracellular matrix. Although initially discovered in fibroblasts, its phosphorylation may also play a role in neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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