Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (MGL3R), PerCP

Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (MGL3R), PerCP

Applications and Tested Dilution

Product Specifications

Additional Formats

• APC
• FITC
• Request custom conjugation

Product Specific Information

A 1:1,000 dilution of MA5-45080 was sufficient for detection of Ubiquitin in 2 µg of purified poly-ubiquitin chains (Poly-Ubiquitin: Ub1-Ub7 WT Chains K63-linked, K48-linked) by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects ubiquitinated proteins, including poly-ubiquitinated proteins (K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins). Does not detect free ubiquitin.

Target Information

Ubiquitin is a highly conserved protein that plays a major role in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein.
This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a.
Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome.
As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

Usage Note

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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