Thermo Fisher Scientific 14-3-3 beta Monoclonal Antibody (03)
Human 14-3-3 beta/YWHAB 단백질을 인식하는 Mouse IgG2b 단클론 항체. ICC/IF, Flow Cytometry, ELISA 등에 사용 가능. 보존제 무첨가, Protein A 정제, 1 mg/mL 농도. 연구용으로만 사용.
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Thermo Fisher Scientific 14-3-3 beta Monoclonal Antibody (Clone 03)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:20–1:100 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| ELISA | 1:1,000–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 03 |
| Immunogen | Recombinant Human 14-3-3 beta/YWHAB protein (Met1–Asn246) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785474 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to avoid contamination.
This antibody specifically recognizes Human 14-3-3 beta/YWHAB.
Target Information
14-3-3 is a family of acidic and soluble proteins highly conserved from yeast to mammals and expressed in all eukaryotic cells. Seven isoforms (beta, gamma, epsilon, eta, zeta, sigma, and tau/theta) are encoded by distinct genes and form homo- and hetero-dimeric cup-shaped structures.
14-3-3 interacts with over 100 binding partners and regulates key proteins involved in signal transduction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology.
Most 14-3-3 interactions require phosphorylation of serine or threonine residues in the target sequence. This protein is abundantly expressed in the brain and has been detected in cerebrospinal fluid of patients with various neurological disorders.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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