Thermo Scientific™

EZ-Link™ Biotin-PEG12-DBCO

EZ-Link Biotin-PEG12-DBCO is a biotinylation reagent that contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry attachment to an azide-labeled자세히 알아보기

EZ-Link Biotin-PEG12-DBCO is a biotinylation reagent that contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry attachment to an azide-labeled molecule, enabling the attachment of biotin using a bioorthogonal approach. The DBCO group reacts preferentially with an azide through a copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) reaction to form a stable triazole linkage with an azide-labeled coupling partner. EZ-Link Biotin-PEG12-DBCO contains a 12-unit polyethylene glycol spacer arm for increased water solubility and reach.

EZ-Link Biotin-PEG12-DBCO features include:
• Bioorthogonal—DBCO and azides react selectively to form triazole linkages
• Enhanced solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
• Irreversible—forms irreversible and stable triazole linkage

Classic click reactions involve a copper-catalyzed azide-alkyne cyclo­addition to label or conjugate molecules. A drawback of this approach is that copper ions can impair protein function, harm cells, and reduce the fluorescence of fluorophores. DBCO is distinct in that it doesn`t require copper to catalyze the reaction with an azide for triazole formation. The strain in the eight-membered ring allows the reaction with azide-modified molecules to occur in the absence of catalysts, preventing copper-induced damage to cells or proteins.

사양

화학물질 반응성Azide

CleavableNo

스페이서 암 길이46.3 Å

배송 조건Wet Ice

제품라인EZ-Link™

라벨링 방법Chemical Labeling

교차결합제 유형Heterobifunctional

반응성 부분DBCO, Biotin

스페이서Long (>30 Å)

수량10 mg

용해도DMSO or DMF

형식Standard

제품 유형Biotin-PEG12-DBCO

Unit SizeEach