Thermo Scientific™

EZ-Link™ DBCO Protein Labeling Kit

The EZ-Link DBCO Protein Labeling Kit provides everything needed to covalently label proteins with a dibenzylcyclooctyne (DBCO) group for copper-free자세히 알아보기

The EZ-Link DBCO Protein Labeling Kit provides everything needed to covalently label proteins with a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry applications. This kit contains the necessary materials to perform five labeling reactions and subsequent purification for removal of excess DBCO reagent. The TFP-PEG4-DBCO provided in this kit readily reacts with the primary amines found on proteins to form a covalently attached DBCO group that can be further reacted with an azide-labeled coupling partner (supplied separately) to create diverse bioconjugates.

EZ-Link DBCO Protein Labeling Kit features include:
• Versatile—label proteins (20–150 kDa) with volumes of 0.5–5 mL
• Chemoselective—DBCO reacts selectively with azides
• Heterobifunctional—reactive towards amine and azides
• Stable—TFP esters are more hydrolytically stable than NHS esters enabling less reagent to be used
• Convenient—labeling and purification can be completed in as little as 90 minutes with 30 min hands-on time

The TFP-PEG4-DBCO provided in this kit is a heterobifunctional crosslinker that contains an amine-reactive tetrafluorophenyl (TFP) group and an azide-reactive dibenzylcyclooctyne (DBCO) group with a water-solubilizing PEG4 spacer in between. In the first conjugation step, the TFP group reacts with a primary amine on a protein to form a stable amide bond and a covalently bound DBCO moiety. Subsequent purification removes excess crosslinker from solution that may interfere with the second labeling step. Once a protein is DBCO-labeled and purified, it can undergo a copper-free strain-promoted azide-alkyne cycloaddition (SPAAC) to form a stable triazole linkage with an azide-labeled coupling partner (supplied separately) for the creation of diverse bioconjugates.

Dibenzylcyclooctynes (DBCO) and azides are bioorthogonal coupling partners as they can react in a biological system without interfering with normal biochemical processes, and as a result there is minimal off-target labeling of macromolecules found in cells or lysates. The reaction between a DBCO and azide can be used for a variety of applications including creation of protein-protein, protein-biomolecule, or protein-small molecule conjugates.
Classic click reactions involve a copper-catalyzed azide-alkyne cyclo­addition to label or conjugate molecules. A drawback of this approach is that copper ions can impair protein function, harm cells, and reduce the fluorescence of fluorophores. DBCO is distinct in that it doesn`t require copper to catalyze the reaction with an azide for triazole formation. The strain in the eight-membered ring allows the reaction with azide-modified molecules to occur in the absence of catalysts, preventing copper-induced damage to cells or proteins.

사양

수량1 Kit

화학물질 반응성Chemical Reactivity

라벨링 방법Chemical Labeling

배송 조건Wet Ice

반응성 부분TFP Ester, DBCO

제품라인EZ-Link™

Unit SizeEach