Thermo Fisher Scientific NBS1 Polyclonal Antibody
Thermo Fisher Scientific의 NBS1 Polyclonal Antibody는 인간 Nibrin 단백질을 인식하는 Rabbit IgG 항체로, WB, IHC, IP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 4°C에서 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific NBS1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 425 and 475 of human Nibrin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex. This protein complex is implicated in Nijmegen breakage syndrome, an autosomal recessive disorder associated with increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity. It provides a direct molecular link between double-strand break repair and cell cycle checkpoint functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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