Thermo Fisher Scientific Phospho-MEK1/MEK2 (Ser221) Recombinant Rabbit Monoclonal Antibody (MEK12S221-D3)
Recombinant rabbit monoclonal antibody recognizing phospho-MEK1/2 (Ser221). Validated for WB and Flow Cytometry. Offers high specificity, lot-to-lot consistency, and animal origin-free formulation. Suitable for research use in human and mouse samples.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Flow Cytometry (Flow) | 1–1,000 ng/mL |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | MEK12S221-D3 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser221 of human phospho MEK1/2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897011 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
MEK1 (MAP Kinase Kinase, also known as MKK) is a key component of the MAP kinase cascade regulating cell growth and differentiation. In the brain, this pathway contributes to synaptic function. Activated MEK1 acts as a dual specificity kinase, phosphorylating both threonine and tyrosine residues on MAP kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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