Takara cDNA amplification kits for 5` and 3` RACE

Overview

SMARTer RACE 5/3 Kit (Cat. # 634858, 634859)

• Simple kit—easy to use
• SeqAmp DNA Polymerase provides robust PCR performance
• In-Fusion HD technology enables fast, easy cloning of RACE fragments
• Requires only 10 ng of total RNA
• Specific enrichment for either 5or 3 ends
• No RNA pretreatment required

Marathon cDNA Amplification Kit (Cat. # 634913)

• Quickly obtain cDNAs without library screening
• Avoid troublesome single-stranded ligation or tailing reactions
• Perform 5and 3 RACE from the same template

More Information

Applications

• RACE (Rapid Amplification of cDNA Ends)
• Identification of 3/5 UTRs
• Full-length gene cloning
• Gene cloning and analysis

References

Bertling, W. M., Beier, F. & Reichenberger, E. Determination of 5` ends of specific mRNAs by DNA ligase-dependent amplification. PCR Methods Appl. 3, 95–9 (1993).

Frohman, M. A. Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: thermal RACE. Methods Enzymol. 218, 340–56 (1993).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.