Thermo Fisher Scientific AMBRA1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Reconstitute in 150 µL of sterile water.
• Centrifuge to remove any insoluble material.
• Specificity: AMBRA1.
• The peptide is homologous in mouse and rat.

Target Information

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, is important for normal growth control and may be defective in tumor cells. It preserves cellular nutrients under starvation and regulates turnover of cytosolic components.
Beclin-1, a principal regulator of autophagosome formation, is regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center, as determined by yeast two-hybrid assay.
Null mutations in this gene in mice cause embryonic lethality with severe neural tube defects due to impaired autophagy, accumulation of ubiquitinated proteins, unbalanced proliferation, and excessive apoptosis.
Down-regulation of Ambra1 via RNA interference reduces rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.