Thermo Fisher Scientific IDH1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human IDH1. Validated for Western blot applications with high specificity. Suitable for research use in cell lines such as HepG2 and HeLa. Supplied in liquid form, stored at 4°C short term or -20°C long term.
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Applications
- Western Blot (WB): 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Human IDH1 (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.45 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735947 |
Product Specific Information
- Positive Control: HepG2, HeLa, Huh-7
- Predicted Reactivity: Rhesus Monkey (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two subclasses: one utilizes NAD(+) and the other NADP(+) as electron acceptors.
Five isocitrate dehydrogenases have been identified: three NAD(+)-dependent (mitochondrial) and two NADP(+)-dependent (one mitochondrial, one cytosolic).
The IDH1 gene encodes the NADP(+)-dependent isocitrate dehydrogenase located in the cytoplasm and peroxisomes, containing a PTS-1 peroxisomal targeting signal.
This enzyme participates in NADPH regeneration for peroxisomal reductions and cytoplasmic NADPH production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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