Thermo Fisher Scientific Apolipoprotein B Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

Apolipoprotein B (apo B) in human plasma is a major protein of low-density lipoproteins (LDL) with a molecular mass of approximately 260–500 kDa. Apolipoprotein B binds to specific receptors on cell membranes and is involved in the removal of LDL and very low-density lipoprotein (VLDL) cholesterol from circulation.

Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls, mediating uptake and clearance of cholesterol from circulation. In atherosclerotic lesions, oxidatively modified LDL is found and recognized by macrophages via scavenger receptor A and CD36. Oxidized LDL may serve as a marker of atherosclerosis.

Apolipoprotein B is synthesized mainly in the liver and is a major apolipoprotein of very low, intermediate, and low-density lipoproteins (LDL), as well as a major component of lipoprotein (a). It acts as a ligand for the LDL receptor, and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/L.

Apolipoprotein B occurs in plasma as two main isoforms: apoB-48 and apoB-100. Both are encoded by a single gene from a single mRNA, with apoB-48 produced through RNA editing of the apoB-100 transcript. Mutations in the Apolipoprotein B gene or its regulatory region cause disorders such as hypobetalipoproteinemia and hypercholesterolemia due to ligand-defective Apolipoprotein B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.