Thermo Fisher Scientific BMF Monoclonal Antibody (OTI3H5), TrueMAB
BMF 단백질을 인식하는 Mouse 모노클로날 항체로 Western blot 및 IHC(P) 검증 완료. Human 시료에 반응하며, 무보존제 동결건조 형태로 제공. 재구성 후 1 mg/mL 농도로 사용 가능하며, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H5 |
| Immunogen | Full length human recombinant protein of human BMF (NP_001003940) produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The BMF protein belongs to the BCL2 family, which regulates apoptosis through hetero- or homodimer formation. It contains a single BH3 domain, binds to BCL2 proteins, and functions as an apoptotic activator.
BMF is sequestered to myosin V motors via association with dynein light chain 2, playing a role in sensing intracellular damage and triggering apoptosis.
Alternative splicing results in multiple isoforms of this protein.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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