Thermo Fisher Scientific SERPINA12 Polyclonal Antibody, MaxPab

Applications

• Western Blot (WB): Tested dilution 1–5 µg/mL

Product Specifications

Product Specific Information

Sequence of this protein is as follows:
MNPTLGLAIF LAVLLTVKGL LKPSFSPRNY KALSEVQGWK QRMAAKELAR QNMDLGFKLL KKLAFYNPGR NIFLSPLSIS TAFSMLCLGA QDSTLDEIKQ GFNFRKMPEK DLHEGFHYII HELTQKTQDL KLSIGNTLFI DQRLQPQRKF LEDAKNFYSA ETILTNFQNL EMAQKQINDF ISQKTHGKIN NLIENIDPGT VMLLANYIFF RARWKHEFDP NVTKEEDFFL EKNSSVKVPM MFRSGIYQVG YDDKLSCTIL EIPYQKNITA IFILPDEGKL KHLEKGLQVD TFSRWKTLLS RRVVDVSVPR LHMTGTFDLK KTLSYIGVSK IFEEHGDLTK IAPHRSLKVG EAVHKAELKM DERGTEGAAG TGAQTLPMET PLVVKIDKPY LLLIYSEKIP SVLFLGKIVN PIGK

Target Information

Vaspin is a newly described adipocytokine expressed predominantly in visceral white adipose tissues. Structure analysis predicts three beta-sheets, nine alpha-helices, and one central loop, characteristic of Serpin family members. Serpins are irreversible serine-protease inhibitors with >30% sequence homology to alpha1-antitrypsin and a conserved tertiary structure containing an exposed reactive center loop acting as a pseudo-substrate.

Members of this family are involved in blood coagulation, fibrinolysis, complement activation, angiogenesis, inflammation, and tumor suppression. In humans, serpins represent ~2% of total serum proteins, with 70% being alpha1-antitrypsin. Vaspin exhibits 40.2% sequence identity with alpha1-antitrypsin, though its protease inhibitory activity is still unknown.

Vaspin mRNA expression in visceral fat correlates positively with BMI and body fat percentage. Administration of vaspin to obese mice improved glucose tolerance and insulin sensitivity, normalizing blood glucose levels and reversing altered expression of diabetes-related adipocytokines (leptin, adiponectin, resistin, TNF-alpha). These findings suggest potential clinical applications for Vaspin in diabetes/obesity metabolic syndrome research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.