Thermo Fisher Scientific TEF1 Recombinant Rabbit Monoclonal Antibody (ARC1157)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

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Immunocytochemistry (ICC/IF)

1:100

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ELISA (ELISA)

1 µg/mL

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Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 400 µg-600 µg extracts of

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ChIP assay (ChIP)

5 µg antibody for 10 µg-15 µg of Chromatin

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CUT&RUN (C&R)

10^5 cells /1 µg

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC1157

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TEAD1 (P28347) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TEF1,uniProtId:P28347-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:426,antigenImageFileName:MA5-35679_TEF1_P28347-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35679_TEF1_P28347-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849579

Product Specific Information

Immunogen sequence: MEPSSWSGSE SPAENMERMS DSADKPIDND AEGVWSPDIE QSFQEALAIY PPCGRRKIIL SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR

Target Information

TEF1 (Transcriptional enhancer factor 1), a member of the TEA/ATTS domain family, is a nuclear protein that is expressed in numerous cell types and plays a role in controlling the expression of numerous genes. TEF family members have a highly conserved DNA-binding domain TEF-1 binds to GT-IIC, SphI/II and M-CAT. TEF-1 also binds to the proximal regulatory element (PRE) of transforming growth factor-alpha, a member of the EGF family that is overexpressed in many types of cancer. Furthermore, TEF-1 represses transcription in placental cells. In vitro, TEF-1 is phosphorylated by several PKC isozymes. TEF-1 is phosphorylated in vivo at serine and threonine residues. Phosphorylation of TEF-1, both in vivo and in vitro, results in a reduction in its DNA-binding capability, which suggests a potential role for TEF-1 in PKC inhibition. TEF-1 also complexes with larger tumor antigen (TAg), and may thus have a role in tumorigenesis. Dimerization of TEF-1 may be important for TEF-1 to function as a regulator of gene transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.