Thermo Fisher Scientific Phospho-PDCD4 (Ser457) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive Control: NIH-3T3 (starvation 16 hours and 5 ng/mL PDGF treatment for 24 hr)
• Predicted Reactivity: Human (100%), Xenopus laevis (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis.

PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung, and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.