Thermo Fisher Scientific YAP1 Polyclonal Antibody
YAP1 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체로, WB, IHC, ICC, IP, ChIP 등 다양한 응용에 적합. 여러 종에 반응하며 Hippo 신호 경로 연구에 유용. 고순도 항원 친화 크로마토그래피로 정제된 액상 형태.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | 8 publications |
| Immunohistochemistry (IHC) | - | 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 1:500 | 4 publications |
| Immunoprecipitation (IP) | 2–10 µg | 1 publication |
| ChIP assay (ChIP) | 1.5 µg/10^6 cells | 3 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat, Zebrafish |
| Published Species | Human, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant YAP1 protein expressed in bacteria |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2219137 |
Product Specific Information
Suggested positive control: brain and kidney lysates, HEK 293 cell lysate, 293 whole cell lysate, antigen standard for YAP1 (transient overexpression lysate), human kidney protein, human brain protein.
Target Information
YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, functioning as a transcriptional co-activator and oncogene. The YAP gene is transcriptionally activated in squamous cell carcinoma cells. YAP acts as both coactivator and corepressor and is a critical downstream regulatory target in the Hippo signaling pathway, which controls organ size and tumor suppression by limiting proliferation and promoting apoptosis. It also plays roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene result in coloboma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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