Thermo Fisher Scientific S100A4/Metastasin/Calvasculin Monoclonal Antibody (S100A4, 1481)
S100A4 단백질을 인식하는 마우스 모노클로널 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등에 사용 가능. 인간 및 마우스 반응성. Protein A/G 정제, PBS 버퍼에 0.05% BSA 및 sodium azide 함유. 연구용으로만 사용.
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Thermo Fisher Scientific S100A4/Metastasin/Calvasculin Monoclonal Antibody (S100A4, 1481)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S100A4, 1481 |
| Immunogen | Recombinant fragment (around aa 2–101) of human S100A4 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, then cool at room temperature for 20 min.
Target Information
The protein encoded by this gene is a member of the S100 family of proteins containing two EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of various cells and are involved in regulating processes such as cell cycle progression and differentiation.
S100 genes include at least 13 members clustered on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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