Thermo Fisher Scientific IL5RA Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-335 of human IL5RA (NP_783855.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IL5RA,uniProtId:Q01344-1,ncbiNodeId:9606,antigenRange:20-335,antigenLength:420,antigenImageFileName:PA5-121210_IL5RA_Q01344-1_Rabbit.svg,antigenImageFileNamePDP:PA5-121210_IL5RA_Q01344-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.54 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914782

Product Specific Information

Positive test controls include: HL-60, B cells, U-87MG, Mouse liver, Mouse lung, Rat lung. The target is usually found in the following locations: Membrane, Single-pass type I membrane protein.

Immunogen sequence: ADLLPDEKIS LLPPVNFTIK VTGLAQVLLQ WKPNPDQEQR NVNLEYQVKI NAPKEDDYET RITESKCVTI LHKGFSASVR TILQNDHSLL ASSWASAELH APPGSPGTSI VNLTCTTNTT EDNYSRLRSY QVSLHCTWLV GTDAPEDTQY FLYYRYGSWT EECQEYSKDT LGRNIACWFP RTFILSKGRD WLAVLVNGSS KHSAIRPFDQ LFALHAIDQI NPPLNVTAEI EGTRLSIQWE KPVSAFPIHC FDYEVKIHNT RNGYLQIEKL MTNAFISIID DLSKYDVQVR AAVSSMCREA GLWSEWSQPI YVGFSR

Target Information

Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cell replacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an alpha chain that binds IL-5 with low affinity and a beta chain that does not bind IL-5, but together with the IL-5 alpha chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the alpha and beta chains is essential for signal transduction. Specifically, the membrane-proximal proline-rich sequence of the cytoplasmic domain of the IL-5R receptor alpha subunit, IL-5Ralpha, is critical for the IL-5 induced proliferative response, expression of nuclear proto-oncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.