Invitrogen™

pRSET A, B, & C Bacterial Expression Vectors

The pRSET vector is designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by자세히 알아보기

The pRSET vector is designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. These cells also produce T7 lysozyme to reduce basal expression of target genes. The pRSET vector offers:

• Bacteriophage T7 promoter for high-level expression
• T7 gene 10 sequence to provide protein stability
• N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody
• N-terminal Xpress™ epitope for detection with the Anti-Xpress™ Antibody
• Enterokinase cleavage site for removal of fusion tag

A set of three vectors is provided (A, B, and C). Each has the N-terminal tag coding sequence in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.

사양

구성 또는 유도성 시스템Inducible

유도제IPTG

프로모터T7

제품 유형Bacterial Expression Vector

선택 제제(진핵)None

항생제 내성 박테리아Ampicillin (AmpR)

분열EK (Enterokinase) Recognition Site

단백질 태그His Tag (6x)

클로닝 방법Restriction Enzyme/MCS

수량20 μg

벡터pRSET

Unit Size20 µg