Invitrogen™

Vybrant™ FLICA Caspase Apoptosis Assay Kits for flow cytometry

Detect apoptosis via flow cytometry with Vybrant FLICA Caspase Assays that detect poly and caspase-3/7/8 activities.

Quickly detect caspase-mediated apoptosis by flow cytometry with Vybrant FAM caspase assay kits for poly-caspases and caspases 3 and 7. The Vybrant FAM caspase assay kits use fluorescent inhibitor of caspases (FLICA) methodology to detect and report caspase activity. Specifically, these caspase assay kits contain engineered FLICA substrates FAM-VAD-FMK (for poly-caspases), FAM-DEVD-FMK (for caspase-3/7), and FAM-LETD-FMK (for caspase-8). They also include Hoechst 33342 and propidium iodide stains, enabling simultaneous evaluation of membrane permeability and cell cycle by flow cytometry.

사양

설명Vybrant FAM Poly Caspases Assay Kit

수량25 assays

형식Tube

키트 구성품1 vial of FAM-VAD-FMK poly caspases reagent (FLICA reagent), 1 vial Hoechst 33342, 1 vial of propidium iodide, 1 vial of DMSO, 1 bottle of apoptosis fixative solution, and 1 bottle of apoptosis wash buffer (10X solution)

제품 유형Caspase Assay Kit

유세포분석기 레이저 라인UV, 488

여기/방출FAM-VAD-FMDK: 488/530
PI: 535/617
Hoechst 33342: 350/461

배송 조건Room Temperature

제품라인Vybrant

검출 방법Fluorescence

용도(장비)Flow Cytometer

라벨 또는 염료FMK, Hoechst 33342, propidium iodide

라벨 유형Other Label(s) or Dye(s)

보관 요구 사항Store in refrigerator (2–8°C) and protect from light.

Unit Size25 assays