Thermo Fisher Scientific beta-2 Adaptin Polyclonal Antibody
Western blot 및 IHC(파라핀)에서 사용 가능한 beta-2 Adaptin에 특이적인 Rabbit Polyclonal Antibody. Human 및 Mouse 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체로, PBS 버퍼에 1% BSA 및 20% 글리세롤 포함. 단기 4°C, 장기 -20°C 보관.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to a region within amino acids 331–937 of Human AP2B1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2546993 |
Product Specific Information
- Recommended positive controls: IMR32, mouse brain
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (99%), Xenopus laevis (98%), Pig (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin-coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane.
There are two characterized adaptors, AP1 and AP2.
AP1 is associated with clathrin-coated vesicles at the trans-Golgi network, and AP2 is associated with endocytic clathrin-coated vesicles at the plasma membrane, shown to interact with Shc and EGF receptor.
AP2 is composed of four subunits: two ~100 kDa gene products (alpha and beta adaptin) and ~50 and ~17 kDa subunits.
There are two alpha-adaptin genes, alpha A and alpha C, which have tissue-specific expression patterns.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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