Thermo Fisher Scientific alanyl-tRNA Synthetase Polyclonal Antibody
Human 및 Mouse 시료에 반응하는 Rabbit Polyclonal 항체로 Western blot 및 IHC(P) 실험에 적합. 항원 친화 크로마토그래피로 정제되었으며 액상 형태로 제공. 단기 4°C, 장기 -20°C 보관 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 461–723 of Human alanyl-tRNA synthetase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.23 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546992 |
Product Specific Information
- Recommended positive controls: A431, H1299, HeLa, HepG2, mouse liver
- Predicted reactivity: Mouse (95%), Rat (92%), Xenopus laevis (84%), Chicken (86%), Bovine (92%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, class II enzymes. These enzymes evolved early and are highly conserved. The 968-residue human AARS shares 41% identity with the E. coli protein.
tRNA synthases interpret the RNA code and attach specific amino acids to tRNAs containing the corresponding anticodons. They consist of a catalytic domain interacting with the amino acid acceptor-TψC helix of the tRNA, and a second domain interacting with the rest of the tRNA structure.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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