Thermo Fisher Scientific Themis Monoclonal Antibody (719945)

Applications

Western Blot (WB)

• Tested Dilution: 1 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 2.5 µg per million cells

Product Specifications

Product Specific Information

• In Western blots, 100% cross-reactivity with recombinant human Themis (aa 2–282) is observed under non-reducing conditions, and approximately 10% cross-reactivity under reducing conditions.
• Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Target Information

Themis is a cytoplasmic protein essential for positive selection and development of single-positive thymocytes. Highest expression is found in CD4+CD8+ double-positive thymocytes, with lower levels in mature T cells. Themis associates with PLCγ1 and LAT, and is phosphorylated by SLP-76 following TCR signaling. There are five isoforms of mouse Themis produced by alternative splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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