Thermo Fisher Scientific Themis Monoclonal Antibody (719945)
마우스 Themis 단백질을 인식하는 Rat IgG2b 모노클로날 항체로, Western blot과 Flow cytometry에 적합합니다. 비결합형으로 동결건조되어 있으며, PBS와 trehalose 완충액에 재구성 가능합니다. 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1 µg/mL
Flow Cytometry (Flow)
- Tested Dilution: 2.5 µg per million cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 719945 |
| Immunogen | E. coli-derived recombinant mouse Themis Lys122–Lys237 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2606930 |
Product Specific Information
- In Western blots, 100% cross-reactivity with recombinant human Themis (aa 2–282) is observed under non-reducing conditions, and approximately 10% cross-reactivity under reducing conditions.
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
Target Information
Themis is a cytoplasmic protein essential for positive selection and development of single-positive thymocytes. Highest expression is found in CD4+CD8+ double-positive thymocytes, with lower levels in mature T cells. Themis associates with PLCγ1 and LAT, and is phosphorylated by SLP-76 following TCR signaling. There are five isoforms of mouse Themis produced by alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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