Thermo Fisher Scientific CHOP Recombinant Rabbit Monoclonal Antibody (7L3A1)
CHOP 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합. 인간, 생쥐, 랫드 반응성. HEK293 세포 발현, 액상 형태, 높은 특이성과 재현성을 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7L3A1 |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human DDIT3/CHOP (NP_004074.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3094182 |
Product Specific Information
Immunogen sequence:
MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG
Target Information
GADD153 (also known as CHOP) is a small nuclear protein that dimerizes with transcription factors C/EBP alpha and beta. This dimer inhibits normal C/EBP binding to classical sites and gains activity on non-classical stress-related targets. Under normal conditions, expression is low but strongly induced during cellular or ER stress, such as tunicamycin treatment, nutrient starvation, or reducing agents affecting calcium flux across the ER membrane.
Additional Information
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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