Thermo Fisher Scientific Phospho-GLI1 (Thr1074) Polyclonal Antibody
GLI1 단백질의 Thr1074 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. Western blot 및 면역세포염색에 적합. 사람, 생쥐, 랫트 반응성. 1 mg/mL 액상 형태로 제공되며, -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human GLI1 (Accession P08151), corresponding to amino acid residues around phosphorylated Thr1074. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817134 |
Product Specific Information
Antibody detects endogenous levels of GLI1 only when phosphorylated at Thr1074.
Target Information
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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