Thermo Scientific™

GeneBLAzer™ MR DA Assay Kit

GeneBLAzer™MR DA(Division Arrested) cells and MR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Mineralcorticoid Receptor (MR)자세히 알아보기

GeneBLAzer™MR DA(Division Arrested) cells and MR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Mineralcorticoid Receptor (MR) fused to the NAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-MR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384- well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both MR DA cells and MRUAS-bla HEK 293T cells are functionally validated for Z` and EC50 concentrations of aldosterone. In addition, MR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and substrate loading time. Additional testing data using alternate stimuli are also available.

사양

대체 작용제AM 580, Fenretinide, 9-cis- retinoic acid, Ch 55, TTNPB

길항제LE 135

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Division-Arrested Cells

검출 방법Fluorescence

유전자 기호MR

일차 작용제All-trans-retinoic Acid

제품라인GeneBLAzer™

수량1 plate

리포터 유전자BLA (Beta-Lactamase)

타겟 입력NR3C2, MR

유형Division-Arrested Cells Assay Kit

세포주293T (HEK)

System TypeGeneBLAzer™

Druggable TargetNuclear Receptors

Unit SizeEach