Thermo Scientific™

GeneBLAzer™ RAR-beta DA Assay Kit

GeneBLAzer™RAR beta DA (Division Arrested) cells and RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human자세히 알아보기

GeneBLAzer™RAR beta DA (Division Arrested) cells and RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RAR beta DA cells and RAR beta-UAS-bla HEK 293T cells are functionally validated for Z` and EC50 concentrations of all-trans retinoic acid (ATRA). In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

사양

대체 작용제Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid

길항제none

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Division-Arrested Cells

검출 방법Fluorescence

유전자 기호RARβ

일차 작용제9-cis-retinoic acid

수량1 plate

리포터 유전자BLA (Beta-Lactamase)

타겟 입력RAR beta, NR1B2, RARB

유형Division-Arrested Cells Assay Kit

세포주293T (HEK)

System TypeGeneBLAzer™

Druggable TargetNuclear Receptors

Unit SizeEach