Thermo Fisher Scientific FGF basic Monoclonal Antibody, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Preparation:
Produced in BALB/c x ICR F1 mice using highly pure recombinant human FGF-basic. Anti-Human FGF-basic-specific antibody was purified from cell culture by Protein G affinity chromatography.

Sandwich ELISA:
In a sandwich ELISA (100 µL/well), 1.0–2.0 µg/mL of this antibody detects ≥125 pg/mL of Recombinant Human FGF-Basic when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human FGF-Basic (500-P18BT) at 0.25–0.50 µg/mL.

Western Blot:
To detect Human FGF-Basic, use at 0.25–0.50 µg/mL. Detection limit for Recombinant Human FGF-Basic is 0.25–0.50 ng/lane under non-reducing conditions.

Target Information

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor family and interacts with high-affinity transmembrane receptors to regulate cell proliferation and tissue neovascularization.
FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is cytosolic and acts via cell surface receptors, whereas the 22–34 kDa isoforms are nuclear and may signal independently of transmembrane receptors.
In humans, the FGF2 gene is located on chromosome 4q and is involved in biological processes such as limb and nervous system development, wound healing, and tumor growth.
FGF2 mRNA includes multiple polyadenylation sites and non-AUG/AUG initiation codons, producing five isoforms. CUG-initiated isoforms are nuclear (intracrine effect), while AUG-initiated forms are cytosolic (paracrine/autocrine effects).
Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.