Thermo Fisher Scientific FGF basic Monoclonal Antibody, PeproTech
FGF-basic 단백질을 인식하는 Mouse Monoclonal 항체로, Western blot 및 ELISA에 최적화됨. E.coli 유래 재조합 Human FGF-basic 항원을 면역원으로 사용. 단백질 G 정제, 동결건조 형태로 제공되며 PBS buffer에 보존. 연구용으로만 사용 가능.
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- 500-M38C-500UG
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | E.coli derived, 17.2 kDa Recombinant Human FGF-basic (154 a.a.) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2942198 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
Preparation:
Produced in BALB/c x ICR F1 mice using highly pure recombinant human FGF-basic. Anti-Human FGF-basic-specific antibody was purified from cell culture by Protein G affinity chromatography.
Sandwich ELISA:
In a sandwich ELISA (100 µL/well), 1.0–2.0 µg/mL of this antibody detects ≥125 pg/mL of Recombinant Human FGF-Basic when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human FGF-Basic (500-P18BT) at 0.25–0.50 µg/mL.
Western Blot:
To detect Human FGF-Basic, use at 0.25–0.50 µg/mL. Detection limit for Recombinant Human FGF-Basic is 0.25–0.50 ng/lane under non-reducing conditions.
Target Information
FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor family and interacts with high-affinity transmembrane receptors to regulate cell proliferation and tissue neovascularization.
FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is cytosolic and acts via cell surface receptors, whereas the 22–34 kDa isoforms are nuclear and may signal independently of transmembrane receptors.
In humans, the FGF2 gene is located on chromosome 4q and is involved in biological processes such as limb and nervous system development, wound healing, and tumor growth.
FGF2 mRNA includes multiple polyadenylation sites and non-AUG/AUG initiation codons, producing five isoforms. CUG-initiated isoforms are nuclear (intracrine effect), while AUG-initiated forms are cytosolic (paracrine/autocrine effects).
Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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