Thermo Fisher Scientific FGF basic Monoclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Immunogen

E.coli derived, 17.2 kDa Recombinant Human FGF-basic (154 a.a.) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FGF2,uniProtId:P09038-4,ncbiNodeId:9606,antigenRange:135-288,antigenLength:288,antigenImageFileName:500-M38C-500UG_FGF2_P09038-4_House_mouse.svg,antigenImageFileNamePDP:500-M38C-500UG_FGF2_P09038-4_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2942198

Product Specific Information

AA Sequence of recombinant protein: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Preparation: Produced in BALB/c x ICR F1 mice using highly pure recombinant human FGF-basic. Anti-Human FGF-basic-specific antibody was purified from cell cµlture by Protein G affinity chromatography.

Sandwich ELISA: In a sandwich ELISA (assuming 100 µL/well), a concentration of 1.0-2.0 µg/mL of this antibody will detect at least 125pg/mL of Recombinant Human FGF-Basic when used with PeproTech`s Biotinylated Antigen Affinity Purified Anti-Human FGF-Basic (500-P18BT) as the detection antibody at a concentration of approximately 0.25-0.50 µg/mL.

Western Blot: To detect Human FGF-Basic by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-Basic is 0.25-0.50 ng/lane, under non-reducing conditions.

Target Information

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.