Thermo Fisher Scientific DNase I Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Immunogen sequence:
SLKIAAFNIQ TFGETKMSNA TLVSYIVQIL SRYDIALVQE VRDSHLTAVG KLLDNLNQDA PDTYHYVVSE PLGRNSYKER YLFVYRPDQV SAVDSYYYDD GCEPCGNDTF NREPAIVRFF SRFTEVREFA IVPLHAAPGD AVAEIDALYD VYLDVQEKWG LEDVMLMGDF NAGCSYVRPS QWSSIRLWTS PTFQWLIPDS ADTTATPTHC AYDRIVVAGM LLRGAVVPDS ALPFNFQAAY GLSDQLAQAI SDHYPVEVML K

Target Information

Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca²⁺/Mg²⁺-dependent endonuclease ubiquitously expressed in mammalian tissues, mediates internucleosomal DNA degradation during apoptosis.
DNase I is highly polymorphic, with at least six known alleles. DNase II, an acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis.
DNase I cleaves DNA to 5′-phosphodinucleotide and 5′-phosphooligonucleotide end-products, whereas DNase II cleaves DNA to 3′-phosphomononucleotide and 3′-phosphooligonucleotide end-products. DNase II is usually cytoplasmic but concentrated in nuclei of lens fibers, while DNase I is concentrated in nuclei of epithelial and fiber cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.