Thermo Fisher Scientific DNase I Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-282 of human DNASE1 (NP_005214.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DNase I,uniProtId:P24855-1,ncbiNodeId:9606,antigenRange:22-282,antigenLength:282,antigenImageFileName:PA5-109650_DNase_I_P24855-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109650_DNase_I_P24855-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.90 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2855061

Product Specific Information

Immunogen sequence: SLKIAAFNIQ TFGETKMSNA TLVSYIVQIL SRYDIALVQE VRDSHLTAVG KLLDNLNQDA PDTYHYVVSE PLGRNSYKER YLFVYRPDQV SAVDSYYYDD GCEPCGNDTF NREPAIVRFF SRFTEVREFA IVPLHAAPGD AVAEIDALYD VYLDVQEKWG LEDVMLMGDF NAGCSYVRPS QWSSIRLWTS PTFQWLIPDS ADTTATPTHC AYDRIVVAGM LLRGAVVPDS ALPFNFQAAY GLSDQLAQAI SDHYPVEVML K

Target Information

Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis. DNase I is highly polymorphic, and at least six alleles of DNase I are known. DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis. DNase I cleaves DNA to 5-phosphodinucleotide and 5-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3-phosphomononucleotide and 3-phosphooligonucleotide end-products. The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts. DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers. In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.