Thermo Fisher Scientific Alpha A Crystallin Polyclonal Antibody
Alpha A Crystallin 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능. 고순도 항원 친화 크로마토그래피로 정제된 리오필라이즈 형태. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 0.5–1 µg/mL |
| Immunohistochemistry (Frozen) (IHC-F) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived human Alpha A Crystallin recombinant protein (Position: M1–S173) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746190 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found in vertebrate skeletal muscle tissue. In the lens, their structural function is to maintain the proper refractive index. The mammalian lens contains three major classes of crystallins: alpha, beta, and gamma.
Alpha-crystallin is the largest and composed of two primary gene products—alpha-A and alpha-B. Alpha-Crystallin comprises about 40% of total lens protein composition and functions as a chaperone-like protein preventing aggregation that may lead to cataract formation. Phosphorylated states of alpha-crystallin occur in response to cellular stress and may play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease, where it accumulates in brain cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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