Thermo Fisher Scientific c-Raf Recombinant Rabbit Monoclonal Antibody (23GB2700)
c-Raf 단백질을 인식하는 토끼 단클론 항체로, Western blot, ICC/IF, Flow cytometry에 적합합니다. HEK293F 발현 시스템에서 생산된 재조합 항체이며, 인간, 마우스, 랫트 반응성을 보입니다. PBS/glycerol 완충액에 보관되며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 23GB2700 |
| Immunogen | A synthesized peptide derived from human Raf1 (300–350 AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Dry ice (international) |
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates MEK1 and MEK2, which subsequently activate ERK1 and ERK2. Activated ERKs regulate gene expression related to cell cycle, apoptosis, differentiation, and migration. Mutations in this gene are linked to Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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