Thermo Fisher Scientific Pellino 1 Polyclonal Antibody
Pellino 1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunocytochemistry에 적합합니다. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS 버퍼에 0.02% sodium azide 포함, 냉장 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 25 µg/mL | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 15 amino acid peptide near the carboxy terminus of human Pellino 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2551817 |
Product Specific Information
- A suggested positive control is human liver tissue lysate.
- PA5-34465 can be used with blocking peptide PEP-1507.
Target Information
The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways leading to activation of MAP kinases and transcription factors.
Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4–IRAK–TRAF6 complex, ultimately resulting in NF-κB activation.
Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase capable of catalyzing the polyubiquitination of IRAK1. It is activated via phosphorylation by IRAK1, IRAK4, or the IKK-related kinases IKK-ε and TBK1. In addition to phosphorylation, Pellino 1 activity is modulated via ubiquitination and sumoylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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