Thermo Fisher Scientific DNA Polymerase beta Polyclonal Antibody
Thermo Fisher Scientific의 DNA Polymerase beta Polyclonal Antibody는 인간, 마우스, 랫트에 반응하며 Western blot, IHC, ICC/IF에 사용 가능. Affinity chromatography로 정제된 액상 형태의 항체로, DNA Polymerase beta 단백질의 내생 수준을 검출. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human POLB (Accession P06746), corresponding to amino acid residues T304–E335 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818097 |
Product Specific Information
Antibody detects endogenous levels of total DNA Polymerase beta.
Target Information
DNA polymerase beta comprises an amino-terminal 8-kDa domain and a carboxy-terminal 31-kDa domain.
The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity, while the C-terminal domain has a nucleotidyltransferase activity.
Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment with DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level.
DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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