Thermo Fisher Scientific HLA-DR/DP Monoclonal Antibody (HL-40)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-dependent

Flow Cytometry (Flow)

• Tested Dilution: 1–5 µg/mL

Immunoprecipitation (IP)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

This antibody reacts with an extracellular common epitope on the beta-chain of human HLA-DR and HLA-DP. DR and DP are isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APCs) such as dendritic cells, B lymphocytes, monocytes, and macrophages.

Target Information

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen-presenting cells (APCs: B lymphocytes, dendritic cells, macrophages).

The alpha chain is approximately 33–35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide-binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5.
[Provided by RefSeq, Jul 2008]

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.