Thermo Fisher Scientific CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Monoclonal Antibody (LPFS2, 4179)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily, including CD40 and CD30. Members of this family regulate cell proliferation and death.
CD27 and its ligand CD70 are mainly expressed in lymphocytes. High CD27 expression depends on antigen receptor ligation, while CD70 expression requires co-stimulatory or pro-inflammatory signals.
CD27 is a disulfide-linked homodimer found on mature thymocytes, peripheral blood T cells, and a subset of B cells. Activation of T cells via TCR-CD3 complex increases CD27 expression and releases soluble CD27 (sCD27), found in plasma, urine, or spinal fluid.
Soluble CD27 serves as a prognostic marker for acute and chronic B cell malignancies. RgpA, a cysteine proteinase, degrades CD27 and counteracts CD27/CD70-mediated T cell activation. CD27-binding protein (SIVA) is a proapoptotic protein implicated in CD27-induced apoptosis.
Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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