Thermo Fisher Scientific Phospho-LIN28A (Ser200) Polyclonal Antibody
LIN28A 단백질의 Ser200 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot 및 Immunocytochemistry에 적합하며, 인간 및 생쥐 시료에 반응. PBS/glycerol buffer에 보관되며, -20°C에서 안정적으로 저장 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human LIN28A (Accession Q9H9Z2), corresponding to amino acid residues around phosphorylated Ser200. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2817124 |
Product Specific Information
Antibody detects endogenous levels of LIN28A only when phosphorylated at Ser200.
Target Information
Lin28 is a transcription factor first identified for its role in developmental timing in C. elegans. It plays a key role in stem cell biology and reprogramming. Expression of Lin28 with transcription factors such as POU5F1, Sox2, and NANOG can induce pluripotent stem (iPS) cells from mouse fibroblasts. Further studies have shown that iPS cells can also be generated using plasmids expressing Lin28, Sox2, POU5F1, and c-Myc, avoiding viral introduction and improving safety for regenerative medicine applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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