Thermo Fisher Scientific EBI3 Monoclonal Antibody (10J811)
Thermo Fisher Scientific의 EBI3 Monoclonal Antibody (Clone 10J811)은 마우스 EBI3 단백질에 특이적인 Rat IgG 항체입니다. Western blot, Flow cytometry, ELISA 등 다양한 어플리케이션에 사용 가능하며, Protein G로 정제된 액상형 제품입니다. -20°C에서 보관하며 연구용으로 적합합니다.
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Thermo Fisher Scientific EBI3 Monoclonal Antibody (10J811)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Flow Cytometry (Flow) | 1.5–3.0 µg/1×10⁶ cells |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10J811 |
| Immunogen | Anti-EBI-3 (RAT) Monoclonal Antibody was produced in rat by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E. coli, followed by hybridoma development. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for mouse EBI3 protein. A BLAST analysis was used to suggest cross-reactivity with EBI3 from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with EBI3 from other species has not been determined.
Target Information
EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a regulatory role in Th2-type immune responses and Th2-mediated tissue inflammation in vivo, possibly through control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form IL-27 and IL-35, respectively. IL-27 is produced by activated antigen-presenting cells upon TLR ligation and negatively regulates Th17 cell differentiation.
EBI3 expression in dendritic cells is regulated by TLR signaling via MyD88 and NF-κB during innate immune responses. It inhibits delayed-type hypersensitivity by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may also contribute to tumor metastasis regulation via lung CD8+ T cells. Targeted deletion of EBI3 protects mice from lung metastasis and reduces Th2 cytokine production associated with allergic airway inflammation in asthma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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