Thermo Fisher Scientific WDR74 Monoclonal Antibody (OTI4B2), TrueMAB
WDR74 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot 및 IHC(P) 분석에 적합합니다. 인간, 마우스, 랫, 개 시료 반응성이 있으며, 무보존제 PBS 버퍼에 동결건조 형태로 제공됩니다. 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4B2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 114–385 of human WDR74 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The yeast nucleolar protein NOP7 is required for the maturation of 66S preribosomes and interacts with multiple proteins. One such protein, NOP seven-associated protein 1 (NSA1), is an essential WD repeat protein involved in yeast 66S ribosome assembly. NSA1 associates with AAA ATPase Rix7, and its release from late nucleolar pre-60S complexes requires Rix7 function. NSA1 is upregulated in mammalian cancer cells, suggesting a role in cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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