Thermo Fisher Scientific CD99 Monoclonal Antibody (M030)
CD99 단백질을 검출하는 Mouse monoclonal 항체(M030)로, Human, Mouse, Rat 시료에 반응합니다. Western blot, ICC/IF, IP 등 다양한 응용에 적합하며, Protein G로 정제된 액상 형태입니다. -20°C에서 보관하며 반복 동결·해동은 피해야 합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M030 |
| Immunogen | Clone M030 was generated from a proprietary antigen related to the extracellular region of human CD99 expressed in the MeWo melanoma cell line. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | ~0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942684 |
Product Specific Information
Clone M030 detects 30 and 32 kDa bands corresponding to the molecular mass of the short and long forms of CD99 on SDS-PAGE immunoblots of human A431, LNCaP, MDA-MB-231, A549, and MeWo cell lysates, as well as mouse LL2 lung carcinoma.
The antibody can be used for multiple applications including ELISA, Western blot, immunocytochemistry, and immunoprecipitation.
In addition, the antibody labels live, unfixed MeWo cells.
Target Information
CD99 (E2, MIC2) is a transmembrane glycoprotein involved in regulation of T cell adhesive properties and programmed cell death distinct from typical apoptosis.
CD99 functions are specific to certain stages of T cell differentiation such as corticothymocytes.
The CD99R isoform expression is restricted in the hematopoietic system to T, NK, and myeloid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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