Thermo Fisher Scientific Phospho-FAK (Tyr397) Polyclonal Antibody

Thermo Fisher Scientific Phospho-FAK (Tyr397) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling.
FAK plays an important role in cell spreading, differentiation, migration, cell death, and the G1 to S phase transition of the cell cycle.
It contains a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, sites where cells attach to the extracellular matrix via integrin receptors.
Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of Grb2 and c-Src PTK with FAK, resulting in ERK2 MAP kinase activation.
In v-Src-transformed NIH3T3 cells, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion. The Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK at Tyr925.
Several transcript variants encoding different isoforms have been identified for the FAK gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.