Thermo Fisher Scientific Phospho-FAK (Tyr397) Polyclonal Antibody
FAK 단백질의 Tyr397 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC 등 다양한 응용 가능. Human, Mouse, Rat 반응성. 고순도 Protein A 정제 및 안정적인 액상 포맷.
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- pk
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Thermo Fisher Scientific Phospho-FAK (Tyr397) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human FAK1 around the phosphorylation site of Tyr397 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling.
FAK plays an important role in cell spreading, differentiation, migration, cell death, and the G1 to S phase transition of the cell cycle.
It contains a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, sites where cells attach to the extracellular matrix via integrin receptors.
Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of Grb2 and c-Src PTK with FAK, resulting in ERK2 MAP kinase activation.
In v-Src-transformed NIH3T3 cells, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion. The Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK at Tyr925.
Several transcript variants encoding different isoforms have been identified for the FAK gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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