Thermo Fisher Scientific alpha-Tubulin Polyclonal Antibody
다양한 종에서 알파 튜불린을 인식하는 폴리클로날 항체로, Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. 인간 알파 튜불린 C-말단 펩타이드로 면역화된 토끼 IgG 항체. 로딩 컨트롤로 사용 가능하며, 광범위한 교차 반응성을 가짐.
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Thermo Fisher Scientific alpha-Tubulin Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,000 |
| ELISA | 1:90,000 |
| Immunoprecipitation (IP) | 1:1,000–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Chicken, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the C-terminal region near amino acids 425–451 of human alpha Tubulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein.
BLAST analysis indicates reactivity with alpha Tubulin from a wide range of organisms including avian, mammalian, aquatic, parasitic, and algal sources based on 100% homology of the immunogen sequence.
Cross-reactivity occurs with all isoforms of alpha Tubulin, making this antibody an excellent loading control.
Target Information
Alpha Tubulin forms a heterodimer with beta Tubulin, each approximately 55 kDa in molecular weight.
This heterodimer is the fundamental unit of microtubules, which are essential structural components in nearly all eukaryotic cells.
Microtubules provide structural support, intracellular transport, and play a key role in mitosis.
Gamma Tubulin assists in microtubule nucleation.
Alpha and beta Tubulin genes are highly conserved among species, with alpha Tubulin closely related to mouse and rat Tuba1 genes.
Expression is predominantly found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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