Thermo Fisher Scientific ergo-1 Polyclonal Antibody, Biotin
C. elegans Ergo1 단백질을 인식하는 Biotin 결합 Rabbit Polyclonal 항체. Western blot, ELISA, Immunoprecipitation에 사용 가능. 고순도 친화 크로마토그래피 정제, 안정적 액상 포맷, 연구용 RNAi 관련 단백질 분석에 적합.
- 카탈로그번호
- ERGO1-BIOTIN
- 판매단위
- pk
카탈로그
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide taken within amino acid region 190–240 on C. elegans Ergo1 protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Argonaute protein required for gene silencing in the endogenous RNA interference (RNAi) pathway. Involved in the 26G RNAi pathway and associates with both unmethylated and methylated 26G small interfering RNAs (26G-siRNAs), a class of 26 nucleotide siRNAs with a guanine residue at the 5′ end. These siRNAs are methylated by the methyltransferase henn-1, which stabilizes them. Association with 26G-siRNAs is required for the biogenesis of secondary 22G-siRNAs, which possess a triphosphorylated guanine residue at the 5′ end. May be involved in passenger strand cleavage of target 26G-siRNAs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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