Thermo Fisher Scientific ergo-1 Polyclonal Antibody, FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1:10,000

-

Immunoprecipitation (IP)

1:50-1:250

-

Product Specifications

Species Reactivity

C. elegans

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide taken within amino acid region 190-240 on C.elegans Ergo1 protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ERGO1,uniProtId:O61931-1,ncbiNodeId:6239,antigenRange:190-240,antigenLength:1121,antigenImageFileName:ERGO1-FITC_ERGO1_O61931-1_Rabbit.svg,antigenImageFileNamePDP:ERGO1-FITC_ERGO1_O61931-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5-1.5 mg/mL

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 7.4-7.8, with 0.5% BSA, 30% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Argonaute protein required for gene silencing in the endogenous RNA interference (RNAi) pathway (PubMed:17110334, PubMed:20133583). Involved in the 26G RNAi pathway and associates with both unmethylated and methylated 26G small interfering RNAs (26G-siRNAs), which are a class of 26 nucleotide siRNAs that possess a guanine residue at the 5-end (PubMed:22102828, PubMed:22548001). Associated 26G-siRNAs are methylated by the methyltransferase henn-1, which stabilizes the siRNAs (PubMed:22548001). Association with 26G-siRNAs is required for the biogenesis of secondary 22G-siRNAs (a class of 22 nucleotide siRNAs that possess a triphosphorylated guanine residue at the 5-end) (PubMed:22102828). May be involved in passenger strand cleavage of target 26G-siRNAs (PubMed:22102828).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.