Thermo Fisher Scientific ergo-1 Polyclonal Antibody, FITC
C. elegans Ergo1 단백질을 인식하는 Rabbit Polyclonal FITC 결합 항체로, Western blot, ELISA, Immunoprecipitation에 적합. 498/517 nm 형광 특성을 가지며, -20°C 어둡게 보관. Affinity chromatography로 정제된 고품질 연구용 항체.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
ELISA
- Tested Dilution: 1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 1:50–1:250
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 190–240 on C. elegans Ergo1 protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Argonaute protein required for gene silencing in the endogenous RNA interference (RNAi) pathway (PubMed:17110334, PubMed:20133583).
Involved in the 26G RNAi pathway and associates with both unmethylated and methylated 26G small interfering RNAs (26G-siRNAs), which are a class of 26 nucleotide siRNAs that possess a guanine residue at the 5′ end (PubMed:22102828, PubMed:22548001).
Associated 26G-siRNAs are methylated by the methyltransferase henn-1, which stabilizes the siRNAs (PubMed:22548001).
Association with 26G-siRNAs is required for the biogenesis of secondary 22G-siRNAs (a class of 22 nucleotide siRNAs that possess a triphosphorylated guanine residue at the 5′ end) (PubMed:22102828).
May be involved in passenger strand cleavage of target 26G-siRNAs (PubMed:22102828).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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