Thermo Fisher Scientific RUNX2 Polyclonal Antibody
RUNX2 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat에 반응합니다. Western Blot, IHC, ICC/IF 등 다양한 응용에 사용 가능하며 항원 친화 크로마토그래피로 정제되었습니다. 골형성 및 골발생 연구용으로 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | – |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | – |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 246–297 of Human RUNX2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803282 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
RUNX2 is a nuclear transcription factor belonging to the RUNX family, containing a Runt DNA-binding domain. It is essential for both membranous and endochondral bone formation, regulating osteoblastic differentiation and skeletal morphogenesis. RUNX2 acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. It binds DNA as a monomer or as part of a heterodimeric complex, enhances TGFBR1 expression in osteoblasts, and cooperates with DLX5 or related factors to activate osteoblast-specific genes. Mutations in RUNX2 are associated with bone development disorders such as cleidocranial dysplasia (CCD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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