Thermo Fisher Scientific ATG14 Polyclonal Antibody
ATG14 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P)에서 사용 가능. HeLa, HepG2 등 다양한 세포주에 적용. 액상 형태로 1 mg/mL 농도, 4°C 단기 보관 및 -20°C 장기 보관 권장. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 159–255 of ATG14 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2552321 |
Product Specific Information
- Recommended positive controls: HeLa, HepG2, 293T, RD, mouse brain, rat brain.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATG14 (Autophagy related protein homolog 14) is encoded by the ATG14 gene and is located on chromosome 14 in humans.
Also known as ATG14L or BARKOR (Beclin 1-associated autophagy-related key regulator).
Autophagy is an evolutionarily conserved process involving recycling of misfolded cellular proteins and degradation of dysfunctional organelles.
It is induced as a cellular response to nutrient stress, leading to the formation of autophagosomes, their fusion with lysosomes, and formation of autophagolysosomes.
The Class III PI3-Kinase, Vps34, and its interacting partner Beclin 1 form a complex that includes ATG14 (type 1) or UVRAG (type 2).
ATG14 guides the type 1 complex to the preautophagosomal structure (PAS) and is critical for the initiation of autophagosome formation.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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